The United States District Court for the Eastern District of Kentucky is the Federal District court whose jurisdiction comprises the following Kentucky counties: Anderson, Bath, Bell, Boone, Bourbon, Boyd, Boyle, Bracken, Breathitt, Campbell, Carroll, Carter, Clark, Clay, Elliott, Estill, Fayette, Fleming, Floyd, Franklin, Gallatin, Garrard, Grant, Greenup, Harlan, Harrison, Henry, Jackson, Jessamine, Johnson, Kenton, Knott, Knox, Laurel, Lawrence, Lee, Leslie, Letcher, Lewis, Lincoln, McCreary, Madison, Magoffin, Martin, Mason, Menifee, Mercer, Montgomery, Morgan, Nicholas, Owen, Owsley, Pendleton, Perry, Pike, Powell, Pulaski, Robertson, Rockcastle, Rowan, Scott, Shelby, Trimble, Wayne, Whitley, Wolfe, and Woodford.
The court is based in Lexington and also in Ashland, Covington, Frankfort, London and Pikeville. In addition, at one time the court also met in Catlettsburg, Richmond and Jackson, however the court left Catlettsburg when the Carl D. Perkins Federal Building and United States Courthouse in Ashland was completed.
The United States Court of Appeals for the Sixth Circuit in Cincinnati, Ohio maintains appellate jurisdiction for the district.
The United States Attorney's Office for the Eastern District of Kentucky represents the United States in civil and criminal litigation in the court.